Differential Activation of Staphylococcus aureus Heme Detoxification Machinery by Heme Analogues

Activation of heme biosynthesis by a small molecule that is toxic to fermenting Staphylococcus aureus.

Staphylococcus aureus is a significant infectious threat to global public health. Acquisition or synthesis of heme is required for S. aureus to capture energy through respiration, but an excess of this critical cofactor is toxic to bacteria. S. aureus employs the heme sensor system (HssRS) to overcome heme toxicity; however, the mechanism of heme sensing is not defined. Here, we describe the id...

Nitrate reductase activity in heme-deficient mutants of Staphylococcus aureus.

Mutants H-14 and H-18 of Staphylococcus aureus require hemin for growth on glycerol and other nonfermentable substrates. H-14 also responds to delta-aminolevulinate. Heme-deficient cells grown in the presence of nitrate do not have lactate-nitrate reductase activity but gain this activity when incubated with hemin in buffer and glucose. Lactate-nitrate reductase activity is also restored to the...

Differential activation of Staphylococcus aureus

3 Catherine A. Wakeman, Devin L. Stauff, Yaofang Zhang, and Eric P. Skaar 4 5 6 7 8 9 10 11 1 Department of Pathology, Microbiology and Immunology, Vanderbilt University School of Medicine, 12 Nashville, TN, USA 37232, 2 Current address: Department of Biology, Grove City College, Grove City, 13 Pennsylvania, USA 16127 14 Running title: Toxic heme analogues activate S. aureus HssRS/HrtAB 15 16

FUNCTION OF NON-HEME-BINDING DOMAINS OF THE STAPHYLOCOCCUS AUREUS ISDB PROTEIN IN HEME ASSIMILATION FROM METHEMOGLOBIN by

Inhibition test of heme detoxification (ITHD) as an approach for detecting antimalarial agents in medicinal plants

Background and objectives: There are several methods to assess the in vitro capability of heme inhibitory activity of antimalarial compounds; most of them require some specific equipment or toxic substances and sometimes the needed materials are not accessible. Regarding the necessity and importance of optimizing and standardizing experimental conditions, the present s...